Fig. 2

Pamidronate restores the inhibited BCL2 and increased cytochrome c in bilirubin-impaired SHED. SHED were cultured as described in Fig. 1a. a–c Sequential expression of BCL2 and cytochrome c (CYC) was analyzed at the indicated time by western blot analysis. Representative images of western blotting were shown (a). Results were shown as the representative expression of BCL2 to beta-actin (ACTB) (b) and CYC to ACTB (c) at each time point in each group. a–c B0, SHED treated with 0 μM bilirubin; B50, SHED treated with 50 μM bilirubin; B50+PAM, SHED treated with 50 μM bilirubin and 10 μM pamidronate. b, c n = 5 for all groups. Statistical analysis was performed as described in the “Methods” section. Graph bars showed the means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.005 (vs. 0 h in each group). NS, no significance