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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Long-term effectiveness of local BM-MSCs for skeletal muscle regeneration: a proof of concept obtained on a pig model of severe radiation burn

Fig. 2

Effect of MSC treatment on deposition of extracellular matrix (ECM) components. a Representative whole-muscle cross-sections stained with Picro Sirius red to identify scar tissue (dark red) and Collagen-3 immunostaining of nonirradiated skeletal muscle (control group), irradiated skeletal muscle on the day of surgery (Irr D100 group), and 12 months after flap surgery with (flap-MSC group) and without (flap group) MSC treatment showed a net reduction of Col3 staining with the MSC treatment. Scale bar 100 μm. b Real-time-PCR of Col3a and Col1a. The flap-MSC group exhibited a net reduction of Col3a and Col1a expression compared with the irradiated and flap groups. Data are expressed relative to control skeletal muscle and normalized to GAPDH. Results are expressed as means ± SEM. P values were calculated by ANOVA with Bonferroni correction, *P < 0.001; compared with nonirradiated skeletal muscle; #P < 0.05; ##P < 0.01; ###P < 0.001compared with irradiated-untreated controls

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