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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human UCB-MSCs treatment upon intraventricular hemorrhage contributes to attenuate hippocampal neuron loss and circuit damage through BDNF-CREB signaling

Fig. 2

hUCB-MSC treatment promotes neuronal survival in the hippocampus through activation of BDNF signaling. a TUNEL staining and immunohistochemistry were performed using sections of paraffin block (P7). DNA was stained with DAPI (blue). TUNEL-positive nuclear staining is green. Neurons were stained with NeuN, a neuronal marker (red). Representative images show the hippocampal CA1 and CA3 regions (left). Scale bar, 50 μm. Quantification of relative immunofluorescence intensity of TUNEL signal is shown as a bar graph (right). Data are shown as mean ± SEM; *p < 0.05 and ** p < 0.005 versus the indicated group (n ≥ 15 per group). b Rat brain paraffin sections were stained with annexin V and NeuN. Bar graph shows the annexin V fluorescence intensity. A representative figure is shown in Additional file 1: Figure S1A. Images shown here are representative of at least three independent experiments. Data are shown as mean ± SEM. *p < 0.05 versus the indicated group. c Whole rat brains were dissected and lysed at the indicated time points and applied for immunoblotting analysis with the indicated antibodies. Bar graph shows quantified amounts of p-TrkB, pAKT, and p-Erk. Data are representative of at least three independent experiments. Data are shown as mean ± SEM; *p < 0.05 and **p < 0.005 versus the indicated group. d Paraffin sections were stained with cleaved caspase3 and NeuN. Bar graph shows the cleaved caspase 3 fluorescence intensity. Data are shown as mean ± SEM from three independent experiments, and a representative image is shown in Additional file 1: Figure S1D. n ≥ 15 per group. **p < 0.005 and ***p < 0.0005 versus the indicated group. ###p < 0.0005 versus N.C, ΦΦΦp < 0.0005 versus IVH

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