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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Human UCB-MSCs treatment upon intraventricular hemorrhage contributes to attenuate hippocampal neuron loss and circuit damage through BDNF-CREB signaling

Fig. 3

hUCB-MSC treatment enhanced neurogenesis in the hippocampus after neuron loss. a, b Effect of MSCs on neurogenesis in the hippocampal dentate gyrus following IVH. At 3 days after severe intraventricular hemorrhage, rat was injected intraperitoneally with 5-bromo-deoxyuridine (BrdU, 100 mg/kg). At 24 h after BrdU i.p. injection, the rat brain was fixed with 4% PFA for 48 h. To determine neurogenesis, a paraffin section was stained with anti-BrdU antibody (green) and DAPI (blue). Scale bar, 100 μm. Data are shown as mean ± SEM; **p < 0.005 versus the indicated group. b Quantification of BrdU+ signal is shown as a bar graph. Images shown here are representative of at least three independent experiments, and each value represents the mean ± SEM of triplicate measurements. *p < 0.05 and **p < 0.005 versus the indicated group. c After severe IVH and transplantation of hUCB-MSCs, P7 rat brain was fixed with 4% PFA for 48 h. Glial cells were stained with anti-GFAP antibody (red). Nuclei were counterstained with DAPI (blue). Scale bar, 100 μm. The bar graph shows GFAP fluorescence intensity. Data are shown as mean ± SEM; n = 3. *p < 0.05 versus the indicated group (right). d Rat hippocampi of IVH/MSC rat models were dissected at p7. Lysates were subjected to IB with the indicated antibodies. Data are representative of three independent experiments. Data are shown as mean ± SEM; n ≥ 15 per group. *p < 0.05 and **p < 0.005 versus the indicated group. ##p < 0.005 versus N.C, ΦΦp < 0.005 versus IVH

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