Fig. 4

The BDNF-Akt/Erk-CREB axis contributes to neurogenesis in the hippocampus after IVH injury. a, b Rat hippocampus were dissected and lysed at p7 in IVH/MSC rat models. Lysates were subjected to IB with the indicated antibodies (left). Amounts of p-TrkB, p-Akt, p-Erk, and p-CREB protein were determined by IB (right). Images shown here are representative of at least three independent experiments, and each value represents the mean ± SEM of triplicate measurements; *p < 0.05 and ** p < 0.005 versus indicated groups, ^##p < 0.005 versus N.C, ^###p < 0.0005 versus N.C, ^Φp < 0.05 versus IVH, ^ΦΦp < 0.005 versus IVH. c Hippocampal slice cultures were prepared after IVH. Slices were cultured for an additional 7 days and fixed with 4% paraformaldehyde. Slice was stained with p-ATK and p-CREB antibody (green). Nuclei were counterstained with DAPI stain (blue). Scale bar, 50 μm. d Rat hippocampus was dissected at P7. RNA was isolated from the hippocampus, and the mRNA levels of rat BDNF were determined using species-specific primers (left). Quantification of rat BDNF mRNA measurements from three independent experiments is shown on the right. Data are shown as mean ± SEM; **p < 0.005 versus the indicated group. ^##p < 0.005 versus N.C, ^ΦΦp < 0.005 versus IVH