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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Human UCB-MSCs treatment upon intraventricular hemorrhage contributes to attenuate hippocampal neuron loss and circuit damage through BDNF-CREB signaling

Fig. 5

hUCB-MSC treatment contributes to the recovery of IVH injury-mediated lesions in the hippocampal trisynaptic circuit. a, b Hippocampal slices of IVH/MSC rat models were cultured at P7. The anterograde axonal tracer biocytin was placed on the entorhinal cortex, D.G, and CA3 at DIV8 and fixed with 4% PFA. Biocytin was visualized using the ABC-DAB method. Red arrows indicate mossy fibers (upper) and Schaffer collateral fibers (bottom). Image shown here is representative of at least three independent experiments. Scale bar, 100 μm. b Bar graph shows mossy fiber intensity and Schaffer collateral fiber intensity. Data are shown as mean ± SEM; n ≥ 20 per group. **p < 0.005 and ***p < 0.0005 versus the indicated group. c To determine the effect of MSCs (BDNF) on hippocampal trisynaptic fiber, MSCs (scr) or MSCs (si-BDNF) were transplanted at p6 following IVH. After 7 days of slice culture, the anterograde axonal tracer biocytin was placed on the slice. Red arrows indicate the mossy (middle) and Schaffer collateral (bottom) fibers. Scale bar, 100 μm. d Bar graph shows mossy fiber intensity and Schaffer collateral fiber intensity. Image shown here is representative of at least three independent experiments. Data are shown as mean ± SEM; **p < 0.005 and ***p < 0.0005 versus the indicated group

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