Fig. 6

Migration behavior of human dermal fibroblasts in AF-MSC-CM (b/−), CM (−s), and CM (b/s). a Optical images of dermal fibroblasts taken from the scratch-wound-closure assay and their relative migration rates. Dermal fibroblasts were synchronized by serum starvation, wounded, and cultured for 18 h. Arrows indicate the wound size. Scale bars, 1 mm. b Relative quantification of ECM molecules (collagen III and MMP1) secreted during dermal fibroblast migration. c Western blot comparison of expression levels of migration factors (vitronectin, syndecan 2, fibronectin, and elastin) in dermal fibroblasts. Error bars represent the mean ± SD of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01