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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Adipose stem cells and their paracrine factors are therapeutic for early retinal complications of diabetes in the Ins2Akita mouse

Fig. 2

Cytokine priming enhances chemokine and angiogenic proteins in ASC-CM and rescues trans-endothelial resistance in vitro. a Assessment of relative protein expression using membrane antibody arrays to determine differential expression of chemokines and angiogenic proteins in unprimed and cytokine-primed ASC-CM. Data represent mean ± SD from one donor ASC performed in triplicates. *p < 0.05; **p < 0.01; ***p < 0.001 compared to unprimed ASC-CM. b Increased levels of IDO, TSG-6, SOD3, SOD2, and IL6 but not TIMP1 proteins in cytokine-primed ASC-CM as assessed by immunoblot. c Representative ECIS tracings plotted as normalized resistance expressed as mean ± SEM from TNF + HG with various study groups. d Quantification of normalized resistance plotted as a reference to untreated control cells at 20 h. Data represented from two independent experiments performed in duplicates. ***p < 0.001 compared to Ctrl; #, p < 0.05, ###, p < 0.001 compared to TNF + HG-treated cultures

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