Fig. 4
Intravitreal injection of ASC or ASC-CM improves retinal function and vision in Ins2Akita mice. a b-wave amplitude measurement in mice at various flash intensities. b b-wave amplitude at 25 cd.s.cm2 expressed as μV. Data represent combined mean ± SEM from n = 12–20 animals/group of the left eye only performed in three separate batches. *p < 0.05 compared to WT mice. c b-wave amplitude measurements in mice at 25 cd.s.cm2 flash intensity were plotted as a ratio of the post-injection b-wave amplitude to the pre-injection b-wave amplitude. Data represent combined mean ± SEM from an n = 8–12 animals/group of both eyes performed in two separate batches. *p < 0.05 compared to WT mice; #p < 0.05 compared to Ins2Akita mice that received saline. d Visual acuity was measured by presenting black and white bars of varying spatial frequencies at 100% contrast, and the contrast sensitivity was measured by changing the gradient that generates tracking at a fixed spatial frequency of 0.042 cycles per degree. Visual acuity in mice expressed as cycles/degree (c/d). e Contrast sensitivity in mice expressed as a percentage. OKN data represent both eyes combined mean ± SEM from n = 6–8 animals/group from a single batch. ***p < 0.001 compared to WT mice and #p < 0.05 compared to Ins2Akita mice that received saline