Fig. 6

Ins2^Akita mice show increased GFAP immunoreactivity partially rescued with ASC-CM. a Representative confocal images showing the GFAP immunostaining (green) counterstained with DAPI (blue) in wildtype animal (WT-Ctrl), Ins2^Akita mice that received saline (Akita-Saline), Ins2^Akita mice that received ASCs (Akita-ASC), and Ins2^Akita mice that received ASC-CM (Akita-ASC-CM). b Quantification of GFAP intensity expressed as average mean integrated intensity measured by NIH ImageJ. Data represent combined mean ± SEM from n = 4–7 eyes/group performed in one batch. *p < 0.02 compared to WT mice and # p = 0.06 compared to Ins2^Akita mice that received saline. Scale = 50 μm