Fig. 5From: Characterization, recellularization, and transplantation of rat decellularized testis scaffold with bone marrow-derived mesenchymal stem cellsIn vitro culture of recellularized scaffolds. MTT assay of the decellularized scaffold revealed that the scaffolds were non-toxic. At the day 1, the viability was significantly lower in the cells seeded on the scaffold compared to those seeded in 2D conventional monolayer cultures. At the days 3 and 7, the proliferation and viability significantly declined in 2D condition, while it significantly elevated in 3D condition. The similar cell viability was recorded in 3D and 2D culture conditions after 3 and 7Â days (a). After 3Â days, the cells within the scaffold showed various phenotypes according to the position (b); those located within the seminiferous tubules were spherical with round nuclei and acidophilic cytoplasm (arrow head), while those located in the interstitium were fibroblast-like (arrow, c). The higher magnification of the seminiferous tubules showed the spherical cells (d). After 7 (e) and 14 (f) days, the cells were present within the scaffolds; however, the cell density declinedBack to article page