Fig. 5
hAESC-derived hepatocyte-like cells improve the liver functions and reduce hepatic damage in ALF mice. a Experimental schematic of CCl4-induced acute liver failure in the NOD-SCID mouse model. The experiments were conducted in three groups including the olive oil group, CCl4+PBS group, and CCl4+HLCs group. b Examinations of liver function. Some liver functional parameters including TBIL, ALT, AST, ALB, and ALP were determined in olive oil, CCl4+PBS, and CCl4+HLC group mice after 3 days and 7 days of HLC transplantation. c H&E staining of liver tissue. HLC injection led to notable improvements in the liver tissue structure at the day 3. d Measurements of necrosis area. The necrosis area of five random, nonoverlapping fields was quantitatively measured in liver tissues of CCl4-induced ALF mice from different experimental groups. Significance was measured via a two-way ANOVA. *P < 0.05, *P < 0.01, ***P < 0.001