Table 1 Primers and conditions used for RT-PCR to detect gene transcripts in hAESCs, HPCs, HLCs, and hPH

GAPDH

F:5′-CCACCCATGGCAAATTCCATGGCA-3′

R:5′-TCTAGACGGCAGGTCAGGTCCACC-3′

Nanog

F:5′-CAATGGTGTGACGCAGGGAT-3′

R:5′-TGCACCAGGTCTGAGTGTTC-3′

OCT4

F:5′-ATCCCTGAACCTAGTGGGGA-3′

R:5′-CACTCGGACCACATCCTTCT-3′

E-cadherin

F:5′-GGAAAATGCCCAGCCCGAC-3′

R:5′-ACGTTATATGCCCGCACGAT-3′

CK7

F:5′-TTTGTGGTGCTGAAGAAGGATG-3′

R:5′-CCACCAGTGGAATTCATCACA-3′

CD105

F:5′-TCCTCCCAAGGACACTTGTA-3′

R:5′-CGCCTCATTGCTGATCATAC-3′

CD73

F:5′-CTCCTCTCAATCATGCCGCT-3′

R:5′-TGGATTCCATTGTTGCGTTCA-3′

CD90

F:5′-GAGGGAGGAAGAGCAGACCT-3′

R:5′-CCTGGATCGGGTTATGATGGG-3′

HLA-DR

F:5′-CAAGAGCTCCAACTATACTCCGAT-3′

R:5′-ACCCTGCAGTCGTAAACGTCC-3′

HLA-ABC

F:5′-GTATTTCTTCACATCCGTGTCCCG-3′

R:5′-GTCCGCCGCGGTCCAAGAGCGCAG-3′

ALB

F:5′-GGTGAGACCAGAGGTTGATGTG-3′

R:5′-GCAGCAGCACGACAGAGTAATC-3′

AAT

F:5′-TGAGTTCGCCTTCAGCCTATACC-3′

R:5′-AGTCCTCTTCCTCGGTGTCCTT-3′

CYP3A4

F:5′-ATGGTCAACAGCCTGTGCT-3′

R:5′-CATGCTGTAGGCCCCAAAGA-3′

CK19

F:5′-GCTTTGTGTCCTCGTCCTCC-3′

R:5′-TTGGCTTCGCATGTCACTCA-3′

CK18

F:5′-ATGAGCTTCACCACTCGCTC-3′

R:5′-TGGCAATCTGGGCTTGTAGG-3′

CK7

F:5′-CCCAGACATCTTTGAGGCCC-3′

R:5′-TTCACGGCTCCCACTCCATC-3′

AFP

F:5′-AGTTTAGCTGACCTGGCTACC-3′

F:5′-GGAGTGGGCTTTTTGTGTGC-3′