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Table 1 Primers and conditions used for RT-PCR to detect gene transcripts in hAESCs, HPCs, HLCs, and hPH

From: Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure

Genes Sequence TM(°C) Size(bp)
GAPDH F:5′-CCACCCATGGCAAATTCCATGGCA-3′ 59 598
R:5′-TCTAGACGGCAGGTCAGGTCCACC-3′
Nanog F:5′-CAATGGTGTGACGCAGGGAT-3′ 52 149
R:5′-TGCACCAGGTCTGAGTGTTC-3′
OCT4 F:5′-ATCCCTGAACCTAGTGGGGA-3′ 59 480
R:5′-CACTCGGACCACATCCTTCT-3′
E-cadherin F:5′-GGAAAATGCCCAGCCCGAC-3′ 60 210
R:5′-ACGTTATATGCCCGCACGAT-3′
CK7 F:5′-TTTGTGGTGCTGAAGAAGGATG-3′ 57 683
R:5′-CCACCAGTGGAATTCATCACA-3′
CD105 F:5′-TCCTCCCAAGGACACTTGTA-3′ 57 244
R:5′-CGCCTCATTGCTGATCATAC-3′
CD73 F:5′-CTCCTCTCAATCATGCCGCT-3′ 55 174
R:5′-TGGATTCCATTGTTGCGTTCA-3′
CD90 F:5′-GAGGGAGGAAGAGCAGACCT-3′ 57 896
R:5′-CCTGGATCGGGTTATGATGGG-3′
HLA-DR F:5′-CAAGAGCTCCAACTATACTCCGAT-3′ 63 273
R:5′-ACCCTGCAGTCGTAAACGTCC-3′
HLA-ABC F:5′-GTATTTCTTCACATCCGTGTCCCG-3′ 65 394
R:5′-GTCCGCCGCGGTCCAAGAGCGCAG-3′
ALB F:5′-GGTGAGACCAGAGGTTGATGTG-3′ 61 695
R:5′-GCAGCAGCACGACAGAGTAATC-3′
AAT F:5′-TGAGTTCGCCTTCAGCCTATACC-3′ 61 530
R:5′-AGTCCTCTTCCTCGGTGTCCTT-3′
CYP3A4 F:5′-ATGGTCAACAGCCTGTGCT-3′ 57 317
R:5′-CATGCTGTAGGCCCCAAAGA-3′
CK19 F:5′-GCTTTGTGTCCTCGTCCTCC-3′ 57 614
R:5′-TTGGCTTCGCATGTCACTCA-3′
CK18 F:5′-ATGAGCTTCACCACTCGCTC-3′ 57 688
R:5′-TGGCAATCTGGGCTTGTAGG-3′
CK7 F:5′-CCCAGACATCTTTGAGGCCC-3′ 57 973
R:5′-TTCACGGCTCCCACTCCATC-3′
AFP F:5′-AGTTTAGCTGACCTGGCTACC-3′ 55 275
F:5′-GGAGTGGGCTTTTTGTGTGC-3′
  1. F forward primer, R reverse primer