Table 2 Primer sequences for real-time quantitative PCR to detect hepatocyte-specific gene transcripts in hAESCs, HLCs, and hPH
Genes | Sequence |
|---|---|
GAPDH | F:5′-CCACATCGCTCAGACACCAT-3′ |
R:5′-GCGCCCAATACGACCAAAT-3′ | |
CYP3A4 | F:5′GCAAGAAGAACAAGGACAACATAGAT-3′ |
R:5′-GCAAACCTCATGCCAATGC-3′ | |
CYP1A2 | F:5′-CTCCTCCTTCTTGCCCTTCA-3′ |
R:5′-TGTAGAAGCCATTCAGCGTTGT-3′ | |
CYP7A1 | F:5′-CTCGAGAGGCCAAAGAAGGA-3′ |
R:5′-CTGTCCAGGCTGGTCTTGAAC-3′ | |
CYP2B6 | F:5′-GAGAGAGTCTACAGGGAGATTGAACA-3′ |
R:5′-GCATTTTGGCTCGGTCATG-3′ |