Fig. 6

Comparison of HE staining for in situ osteogenesis of osteogenic micro-tissue and digested cells. In the upper, middle, and lower sections of the MT group (a, d, g), there was a significant bone-like structure (black arrow) in the scaffold. The bone-like structure was also found in the digested cell group (b, e, h), but the area was relatively small. In the blank control group (c, f, i), only a small amount of new bone was ingrowth into the upper part, and no obvious bone formation was observed in the other sections. j Vascularized area/tissue area (VA/TA, %) of the osteogenic micro-tissue, digested cells, control groups. The blank “round hole” is a space occupied by PCL scaffold (bar 500 μm). MT osteogenic micro-tissue, DC, digested cells; Control, control group