Fig. 2

Mesenchymal stem cell culture. RFP⁺ MSCs were cultured in the absence (A, A′) or presence of TGFß1 (B, B′) for 15–21 days. MSCs showed a spindle-shaped morphology (A and B bright field in; A′ and B′ fluorescence microscope with Texas red filter). TGFß1-treated MSCs occasionally showed spheroid-like colonies. MSCs were positive for CD44 (C), SCA1 (D), and CD29 (E) and negative for CD45 (F). (G) Cytokine secretion profiles were obtained by incubating antibody array membranes with conditioned media from TGFß1-treated and non-treated MSC cultures (at 3 weeks). A, A′, B, B′ Scale bar = 200 μm. C–F Scale bar = 100 μm