Fig. 1
From: Influences of donor and host age on human muscle-derived stem cell-mediated bone regeneration
In vitro osteogenesis of young and old donor hMDSCs. a BMP2 secretion levels of 6 populations of LBMP2/GFP-transduced cells. b MicroCT 3D images of pellet culture for non-transduced and LBMP2/GFP-transduced hMDSCs. LBMP2/GFP-transduced hMDSCs showed larger mineralized pellets in all groups. c Quantification of mineralized pellet volume showed significantly higher mineralized pellet volume in all LBMP2/GFP-transduced cells compared to each respective non-transduced hMDSC counterpart. Young donor 1 LBMP2/GFP-transduced cells formed larger pellets than old donor 1 LBMP2-transduced cells. Young donor 2 LBMP2/GFP-transduced hMDSCs also formed larger pellets than old donor 2 cells. No significant difference was found between young donor 3 and old donor 3 LBMP2/GFP-transduced cells. Young donor 2 non-transduced hMDSCs also formed larger mineralized pellets than old donor 2 non-transduced hMDSCs. d Von Kossa staining showed that LBMP2/GFP-transduced hMDSCs had more mineralization (as shown in black) than did non-transduced cells. e Osteocalcin immunochemistry revealed the osteogenic differentiation of both non-transduced and LBMP2/GFP-transduced hMDSCs in all groups. Note that highly mineralized parts of the cell pellets often peeled away. *P < 0.05, ***P < 0.001