Fig. 5

Comparison of bone regeneration in young and old animal hosts after transplantation of old donor LBMP2/GFP-transduced hMDSCs. a MicroCT 3D images of skull defect injury and new bone volume quantification using old donor 1–3 cells. b New bone volume was significantly lower in old hosts than in young hosts at 4 and 6 weeks post-injury after old donor 1 cells were transplanted. c No difference was found for new bone volume in young and old hosts at any time point when old donor 2 cells were used. Of note, the new bone volume regenerated using old donor 2 cells was smaller than that using the other two old donor cell populations (old donor 1 and old donor 3) at respective time points. d New bone volume was significantly lower in old hosts compared to young hosts at all time points measured when old donor 3 cells were used. e When results for all three old hosts were combined and compared to combined results for all three young hosts, new bone volume was significantly lower in old hosts at 2, 4, and 6 weeks post-injury. *P < 0.05, **P < 0.01