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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Influences of donor and host age on human muscle-derived stem cell-mediated bone regeneration

Fig. 6

Representative Herovici’s and H&E staining of regenerated bone. a Herovici’s staining showed positive bone matrix collagen type I (Col1, stained pink red; bone marrow [BM] light blue and collagen type3 [Col3], blue) in the defect area in all groups. All groups stained positive for Col1, as displayed by the trabecular-like red structure, when young and old donor cells were transplanted into young or old hosts. However, new bone formed in old animal hosts was less dense than in young hosts. The area between two black arrows in each picture shows the defect area (× 200 is the box area from × 20). b H&E staining indicated that the new bone in all groups was functional bone, as the defect area observed for all groups showed both bone matrix (black arrows in all pictures that point to the tissues other than bone marrow) and bone marrow, with hematopoietic stem cells as well as blood vessels. Insets are the enlarged black boxes in each picture that indicate megakaryocytes. Yellow arrows indicate red blood cells, and blue arrows indicate myeloid cells

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