Fig. 9
From: Influences of donor and host age on human muscle-derived stem cell-mediated bone regeneration
Western blot and qRT-PCR analysis. a, b Western blot of phosphorylated p38MAPK (pp38MAPK) levels. Old hMDSCs expressed significantly less pp38MAPK compared to young hMDSCs. c, d Western blot images and quantification revealed no significant differences for the expression of p16IN4a (senescence marker) between young and old hMDSCs. e, f Western blot images and quantification of pAKT (cell survival marker). No significant differences were observed between young and old hMDSCs. g Quantitative RT-PCR (qRT-PCR) analysis of mRNA expression of young and old hMDSCs. COX2 expression was significantly lower (high delta CT) in old hMDSCs than in young hMDSCs. h qRT-PCR analysis of young and old LBMP2/GFP-transduced hMDSCs. GPX1 expression was increased, while COX2 expression was decreased in old compared to young LBMP2/GFP-transduced cells. *P < 0.05. **P < 0.01