Table 2 Growth medium for MenSCs
From: Human menstrual blood: a renewable and sustainable source of stem cells for regenerative medicine
| Â | Components | Reference | ||||
|---|---|---|---|---|---|---|
Base | FBS (v/v) | Antibiotics | Other nutrients | Growth factors | ||
Protocal 1 | DMEM | 20% | 1% penicillin and streptomycin | 1% glutamine | Â | [25] |
Protocal 2 | DMEM/F12 | 10% | 100Â U/mL penicillin and 100Â mg/mL streptomycin | Â | Â | |
Protocal 3 | Basic MSU-1 | 5% | Â | 2Â mM N-acetyl-l-cysteine, 0.2Â mM l-ascorbic acid-2-phosphate | Â | [74] |
Protocal 4 | Minimal alpha GutaMAX | 10% | 10,000 U/mL penicillin, 10 mg/mL streptomycin and 25 μg/mL amphotericin in 0.9%NaCl | 20 mmol/L N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid pH 7.2–7.4 | 1.6 nmol/mL prednisolone sodium phosphate 80 μU/mL insulin 20 ng/mL FGF | [75] |