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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: In vitro and in vivo potentialities for cartilage repair from human advanced knee osteoarthritis synovial fluid-derived mesenchymal stem cells

Fig. 3

Histological, immunohistochemical analyses and GAG contents of cartilage engineered substitutes produced using human SF-MSCs and collagen sponge at D28 under various oxygen conditions and various growth factors. All observations were carried out on three different sponges for each culture condition and for each patient. The differences between three sponges of each group are very low; we choose to submit only one photograph. The sections were stained with alcian blue to reveal proteoglycan content (a) and immuno-histochemical analyses against type II collagen (Col2) was performed (c). The scale bars represent 400 μm. b and d represents densitometry measurement of alcian Blue and type II collagen respectively. Concentration of GAGs was measured inside sponges with a colorimetric assay using dimethylmethylene blue (e). Statistical analysis was performed initially with a one-way ANOVA with a Dunnett’s post hoc test versus their internal control ITS for each condition (normoxia separated from hypoxia) followed by a two-way ANOVA with a Bonferroni’s post hoc test on all the values the interaction of hyopoxia. Asterisks represent significative difference versus the control condition (ITS 1%) *p < 0.05; **p < 0.01; ***p < 0.001 (c, d, and e). There is no significant interaction of hypoxia

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