Gene | Primer sequence | Annealing temperature (°C) | Amplicon size (bp) | Accession number | |
---|---|---|---|---|---|
RPS29 | Fwd | 5′-AGATGGGTCACCAGCAGCTGTACTG-3′ | 60 | 73 | NM_001032 |
Rev | 5′-AGACACGACAAGAGCGAGAA-3′ | ||||
COL2A1 | Fwd | 5′-ATGACAATCTGGCTCCCAAC-3′ | 55 | 200 | NM_001844 |
Rev | 5′-GAACCTGCTATTGCCCTCTG-3′ | ||||
COL2B | Fwd | 5′-GCATGAGGGCGCGGTAGAGA-3′ | 70 | 195 | NM_033150.2 |
Rev | 5′-TGGTCCTGGTTGCCGGACAT-3′ | ||||
COL1A1 | Fwd | 5′-AGGTGCTGATGGCTCTCCT-3′ | 60 | 104 | NM_000088.3 |
Rev | 5′-GGACCACTTTCACCCTTGT-3′ | ||||
ACAN (Aggrecan) | Fwd | 5′-TCGAGGACAGCGAGGCC-3′ | 63 | 85 | NM_001135 |
Rev | 5′-TCGAGGGTGTAGCGTGTAGAGA-3′ | ||||
VCAN (Versican) | Fwd | 5′-TGTTCCTCCCACTACCCTTG-3′ | 62 | 122 | NM_001164098 |
Rev | 5′-CTTCCACAGTGGGTGGTCTT-3′ | ||||
COMP | Fwd | 5′-ACAATGACGGAGTCCCTGAC-3′ | 60 | 115 | NM_000095 |
Rev | 5′-TCTGCATCAAAGTCGTCCTG-3′ | ||||
SOX9 | Fwd | 5′-GAGCAGACGCACATCTC-3′ | 55 | 118 | NM_000346 |
Rev | 5′-CCTGGGGATTGCCCCGA-3′ | ||||
RUNX2 | Fwd | 5′-CCCGTGGCCTTCAAGGT-3′ | 60 | 73 | NM_001278478 |
Rev | 5′-CGTTACCCGCCATGACAGTA-3′ | ||||
BGLAP (OSTEOCALCIN) | Fwd | 5′-GTGCAGAGTCCAGCAAAGGT-3′ |  |  |  |
Rev | 5′-TCAGCCAACTCGTCACAGTC-3′ | 62 | 175 | NM_199173 |