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Table 1 Primers used for RT-qPCR

From: In vitro and in vivo potentialities for cartilage repair from human advanced knee osteoarthritis synovial fluid-derived mesenchymal stem cells

Gene

Primer sequence

Annealing temperature (°C)

Amplicon size (bp)

Accession number

RPS29

Fwd

5′-AGATGGGTCACCAGCAGCTGTACTG-3′

60

73

NM_001032

Rev

5′-AGACACGACAAGAGCGAGAA-3′

COL2A1

Fwd

5′-ATGACAATCTGGCTCCCAAC-3′

55

200

NM_001844

Rev

5′-GAACCTGCTATTGCCCTCTG-3′

COL2B

Fwd

5′-GCATGAGGGCGCGGTAGAGA-3′

70

195

NM_033150.2

Rev

5′-TGGTCCTGGTTGCCGGACAT-3′

COL1A1

Fwd

5′-AGGTGCTGATGGCTCTCCT-3′

60

104

NM_000088.3

Rev

5′-GGACCACTTTCACCCTTGT-3′

ACAN (Aggrecan)

Fwd

5′-TCGAGGACAGCGAGGCC-3′

63

85

NM_001135

Rev

5′-TCGAGGGTGTAGCGTGTAGAGA-3′

VCAN (Versican)

Fwd

5′-TGTTCCTCCCACTACCCTTG-3′

62

122

NM_001164098

Rev

5′-CTTCCACAGTGGGTGGTCTT-3′

COMP

Fwd

5′-ACAATGACGGAGTCCCTGAC-3′

60

115

NM_000095

Rev

5′-TCTGCATCAAAGTCGTCCTG-3′

SOX9

Fwd

5′-GAGCAGACGCACATCTC-3′

55

118

NM_000346

Rev

5′-CCTGGGGATTGCCCCGA-3′

RUNX2

Fwd

5′-CCCGTGGCCTTCAAGGT-3′

60

73

NM_001278478

Rev

5′-CGTTACCCGCCATGACAGTA-3′

BGLAP (OSTEOCALCIN)

Fwd

5′-GTGCAGAGTCCAGCAAAGGT-3′

   

Rev

5′-TCAGCCAACTCGTCACAGTC-3′

62

175

NM_199173