Fig. 4

Inhibition of autophagy promotes proliferation of MenSCs. a Representative WB of ATG5 in MenSCs infected with lentivirus expressing shGFP or shATG5. t test, ***p < 0.001. b WB was used to detect autophagy proteins, LC3, Beclin1, and p62 in MenSCs after infected with lentivirus expressing shGFP or shATG5. t test, **p < 0.01 and ***p < 0.001. c Cell proliferation assay between the shGFP and shATG5 groups using MTT assay. t test, ***p < 0.001. d Cell proliferation with chloroquine at different concentration was assessed using MTT assay, one-way ANOVA followed by Dunnett’s test, **p < 0.01 and ***p < 0.001, versus 0 μM CQ. e WB was used to detect Cdk2 and Cdk4 expression in shGFP or shATG5 MenSCs. t test, **p < 0.01. f Real-time PCR analysis of P21 and P16 mRNA expression in shGFP or shATG5 MenSCs. t test, **p < 0.01 and ***p < 0.001. All data are provided as means ± SEM