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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Autophagy induces G0/G1 arrest and apoptosis in menstrual blood-derived endometrial stem cells via GSK3-β/β-catenin pathway

Fig. 6

Autophagy inhibits cell proliferation through the GSK3β/β-catenin pathways in MenSCs. af WB was used to detect p-Gsk3β, GSK3β, and β-catenin in MenSCs after treatment in different combinations, one-way ANOVA followed by Dunnett’s test, *p < 0.05, **p < 0.01, and ***p < 0.001, as compared to the left column. g WB of GSK3β in MenSCs infected with lentivirus expressing shGFP or shGSK3β. t test, ***p < 0.001. h, i Cell proliferation was assessed between shGFP and shGSK3β using MTT assay. t test, **p < 0.01. j The proliferation capacity during the 24-day culture was determined by MTT assay in MenSCs treated with CHIR99021, one-way ANOVA followed by Dunnett’s test, *p < 0.05 and ***p < 0.001, versus DMSO. km WB of p-Gsk3β, GSK3β, and β-catenin in shGFP or shGSK3β MenSCs after treatment. Statistical analysis is based on one-way ANOVA followed by Tukey test; ns represents not significant, *p < 0.05,**p < 0.01, and ***p < 0.001. All data are provided as means ± SEM

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