Fig. 3

MEIS2 deletion impairs early hematopoiesis of hESCs. a Representative morphology of “cobblestone-like” cells generated from WT, MEIS2^+/−, and MEIS2^−/− hESCs at day 7 of hematopoietic differentiation. The “cobblestone-like” cells were indicated by orange arrows. Scale bar, 50 μm. b Immunofluorescence of WT, MEIS2^+/−, and MEIS2^−/− hESCs with antibody against CD43 (red) at day 7 of hematopoietic differentiation. Nuclei were stained with DAPI (blue). Scale bars, 50 μm. c Flow cytometry analysis of CD43⁺ hematopoietic precursors generated from WT, MEIS2^+/−, and MEIS2^−/− hESCs at day 7 of differentiation. d Flow cytometry analysis of CD45⁺ hematopoietic cells generated from WT, MEIS2^+/−, and MEIS2^−/− hESCs at day 11 of differentiation. e The total number of colonies derived from WT, MEIS2^+/−, and MEIS2^−/− hESCs (left). The proportion of BFU-E, CFU-E, CFU-GM, and CFU-GEMM in total colonies. Results are shown as means ± SEM (n = 3). NS not significant; *P < 0.05, **P < 0.01, and ***P < 0.001