Fig. 5

MEIS2 deletion impairs early hematopoietic differentiation from hESCs by suppressing HEP specification and EHT. a GSEA depicting the enriched processes in “endothelium development” and “hematopoietic cell lineages.” b Heatmap of 93 downregulated genes in differentiated cells from MEIS2^−/− hESCs compared with WT. Endothelium specification and hematopoiesis associated genes are listed on the right. c Expression of GATA2 and TAL1 in differentiated cells from WT and MEIS2^−/− hESCs at day 5 of differentiation with real-time PCR analysis. d Flow cytometry analysis of CD43⁺ hematopoietic precursors generated from WT and MEIS2^−/− hESCs at day 7 of differentiation with or without exogenous expression of TAL1. The analysis was performed by gating GFP⁺ cells. e Flow cytometry analysis of CD31⁺ CD34⁺ HEPs generated from WT and MEIS2^−/− hESCs at day 5 of differentiation with or without exogenous expression of TAL1. The analysis was performed by gating GFP⁺ cells. f GSEA profiles show the enrichment of genes expressed differentially between WT and MEIS2^−/− hESCs and those expressed differentially in “TAL1-activated genes in FLK+ MES (mesoderm)” (left) and TAL1 WT vs TAL1^−/− gene sets (right). Results are shown as means ± SEM (n = 3). NS not significant; *P < 0.05, **P < 0.01, and ***P < 0.001