Fig. 6

MEIS2 is dispensable for megakaryopoiesis and thrombopoiesis. a Representative morphologies of differentiated WT and MEIS2^−/− hESCs at day 3 of megakaryocytic differentiation. Large cells are indicated by orange arrows. Scale bar, 20 μm. b Flow cytometry analysis of CD41a⁺CD42b⁺ megakaryocytes at day 3 of megakaryocytic differentiation. c Distribution of diameter of 100 cells in each group. The sizes of cells were measured by using Nano software. d Flow cytometry analysis of the cell size in WT and MEIS2^−/− hESCs at day 3 of megakaryocytic differentiation. e Representative morphologies of proplatelets generated from WT and MEIS2^−/− hESCs at day 6 of megakaryocytic differentiation. Scale bar, 20 μm. f Flow cytometry analysis of CD41a⁺CD42b⁺ proplatelets at day 6 of megakaryocytic differentiation. MEIS1^−/− hESCs were used as a control. Results are shown as means ± SEM (n = 3). NS not significant; *P < 0.05, **P < 0.01, and ***P < 0.001