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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Suppression of AKT-mTOR signal pathway enhances osteogenic/dentinogenic capacity of stem cells from apical papilla

Fig. 3

Phosphorylation of AKT and mTOR and expression of RUNX2, BGLAP, and DSPP in AKT-siRNA-, LY294402-, and rapamycin-pretreated SCAP under in vitro osteogenic/dentinogenic differentiation. SCAP were pretreated with or without AKT-siRNA (20 nM)- (a–c), LY294402 (50 μM)- (d, e), and rapamycin (100 nM) (f, g) and were cultured under osteogenic/dentinogenic condition (Os/Den) for 1 week. The expression of AKT, p-AKT, mTOR, p-mTOR, RUNX2, BGLAP, and DSPP was examined by western blot analysis and were shown in the Fig. 2a, b, e, f, i, j. a, b, d, f Relative expression of AKT/ACTB (a), p-AKB/ACTB (b), p-AKB/AKB (d), and p-mTOR/mTOR (f) in SCAP. c, e, g Relative expression of RUNX2, BGLAP, and DSPP in SCAP. ag n = 5 for all groups. *P < 0.05, **P < 0.01, ***P < 0.005. Graph bars show the means ± SEM

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