Fig. 4

Effects of LY294402 and rapamycin on in vivo osteogenic/dentinogenic differentiation of SCAP. SCAP were pretreated with or without LY294402 (50 μM) and rapamycin (100 nM) and were subcutaneously transplanted into immunocompromised mice. a Representative images of the transplant tissues (TP) by hematoxylin and eosin staining (HE). HA, HA/TCP carriers. b Measurement of the area of de novo calcified tissues in the transplant tissues. c RT-qPCR analysis determines the expression of RUNX2, BGLAP, and DSPP in the transplant tissues. Results are shown as the ratios to the non-pretreated SCAP-transplant group. a, c Intact, non-pretreated SCAP-transplanted group. b, c: n = 5 for all groups. *P < 0.05 and ***P < 0.005. Graph bars show the means ± SEM