Fig. 2
The prepared TSCM cells bear the ability of self-renewing. a TN, TSCM, TCM, and TEM cell subsets in CD8+ T cells were sorted by their corresponding phenotypes from the co-culture on day 7. TREC copy number in the sorted subsets relative to TN cells was shown. Data are represented as mean ± SD of four individual experiments. b The prepared TSCM cells were relabeled with CFSE and exposed to 200 U/ml of IL-2 for 10 days, the proliferating daughter cells divided into 7 generations (left panel). In each generation, there were a portion of CD8+ T cells expressing CD62L and CD45RA (right panel). Representative FCM plots were shown. c The frequency of TSCM (CD45RA+ CD62L+) cells in CD8+ T cells was analyzed at each CFSE dilution peak. Data are represented as mean ± SD of four individual experiments. (*p < 0.05; **p < 0.01, and NS, p > 0.05)