Fig. 3From: Antigen-specific CD8+ memory stem T cells generated from human peripheral blood effectively eradicate allogeneic targets in miceE007 cells stimulate the TSCM differentiate rapidly and the daughter effectors produce cytokines. The prepared TSCM were stimulated with E007, E001, and α-CD3/CD28, respectively. a, b The TSCM differentiated into effector T cells upon the stimulation with E007. CD3+ CD8+ T cells expressing CD45RA and CD62L (TSCM cells) were detected at indicated hours after stimulation. Representative FCM plots were shown (a). Data are represented as mean ± SD of four individual experiments (b). c–f Effector T cells differentiated from the TSCM produced cytokines. Intracellular IL-2, TNF-α, and IFN-γ production of the T cell subsets were detected. Gating by CD62L, the T cells were divided into CD62L+ (TSCM and TCM) and CD62L- (TEM and TEF) cells, the TEM and TEF subsets showed the cytokine positively stained cells. Representative FCM plots (the percentages indicated the cytokine producing cells in CD62L+ and CD62L- cells, respectively) (c) and the frequencies of IFN-γ (d), TNF-α (e) and IL-2 (f) positive cells in corresponding CD8+ T cell subsets were shown. Data are represented as mean ± SD of four individual experiments (**p < 0.01; ***p < 0.001, and NS, p > 0.05)Back to article page