Fig. 3
From: A pH probe inhibits senescence in mesenchymal stem cells
Changes of senescence-associated markers showed that SGJ suppressed cellular aging and promoted cell proliferation. a SA-β-gal activity and morphology of young (PDL 6) and senescent (PDL 22) BMSCs. Normal BMSCs at PDL 6 and PDL 22. Senescent BMSCs treated with 1, 5, 10, and 20 μM SGJ for 48 h. Percentage of SA-β-gal-positive cells. b Immunofluorescence assay of SAHF and its components HP1-γ and H3K9Me2/3 in young (PDL 6) and senescent (PDL 22) BMSCs treated with/without SGJ (20 μM) for 48 h. The arrow indicating dots are those co-localize with corresponding dots in different channels. c Western blot assay of p21 indicated a decline of senescent protein by treating with 1, 5, 10 and 20 μM SGJ for 48 h in senescent BMSCs. d Effect of treatment with SGJ (20 μM) for 48 h on proliferation of senescent BMSCs (PDL 22) analyzed by flow cytometry. e Cell viability in BMSCs at PDL 22 with 1, 5, 10 and 20 μM SGJ for 48 h. (*, p < 0.05; **, p < 0.01, results were expressed as means ± SEM, n ≥ 3)