Fig. 4

Identification of ASCs and effect of HIF1A-AS2 on ASC osteogenic differentiation. a Flow cytometry for CD29/CD31/CD44/CD45 detection. b qRT-PCR: expression of HIF1A-AS2 in normal/induced ASCs over time. c qRT-PCR: expression of HIF1A-AS2 in induced ASCs after pcDNA3.1-HIF1A-AS2 or sh-HIF1A-AS2 transfection. d qRT-PCR: expression of Runx2, Osterix, and Osteocalcin after transfection. e Western blot analysis of Runx2, Osterix, and Osteocalcin after transfection. f Alizarin red staining (21 days) and ALP staining (14 days): changes of calcium nodule formation and ALP activity after transfection. ^* P < 0.05, ^** P < 0.01, compared with NC group; ^# P < 0.05, ^## P < 0.01, compared with sh-NC group