Skip to main content
Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: RETRACTED ARTICLE: Long non-coding RNA HIF1A-AS2 facilitates adipose-derived stem cells (ASCs) osteogenic differentiation through miR-665/IL6 axis via PI3K/Akt signaling pathway

Fig. 7

Effects of HIF1A-AS2/miR-665/IL6 axis on the PI3K/Akt signaling pathway. a, b Western blot: effects of pcDNA3.1-HIF1A-AS2/pcDNA-IL6/agomiR-665 and PI3K inhibitor LY294002 (10 μM) on expression of PI3K/Akt-related proteins IL6/IL6R/Akt/p-Akt, reflecting the activation of the PI3K/Akt signaling pathway. c Alizarin red staining (21 days) and ALP staining (14 days): changes of calcium nodule formation and ALP activity after pcDNA3.1-HIF1A-AS2/pcDNA3.1-IL6/agomiR-665 transfection or LY294002 treatment. * P < 0.05, ** P < 0.01, compared with NC group; # P < 0.05, ## P < 0.01, compared with sh-NC group

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512