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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Overexpression of apelin in Wharton’ jelly mesenchymal stem cell reverses insulin resistance and promotes pancreatic β cell proliferation in type 2 diabetic rats

Fig. 3

Detection of overexpression of apelin in transduced WJ-MSCs. a Western blot analysis shows strong apelin band in the WJ-MSCs transduced with apelin expressing lentiviral particles compare to the vector lentiviral particles or nontransduced WJ-MSCs. The intracellular GAPDH protein are used as a loading controls; b ELISA analysis of the apelin level in the cell culture media of apelin-transduced WJ-MSCs compared to the media of the nontransduced WJ-MSCs. c, d Quantitative analysis of apelin expression level of mRNA via RT-PCR of apelin in the apelin-transduced cells, vector-transduced cells, and the nontransduced WJ-SCs. The values are mean ± SD from four individual experiments. **P < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512