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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Type I collagen deposition via osteoinduction ameliorates YAP/TAZ activity in 3D floating culture clumps of mesenchymal stem cell/extracellular matrix complexes

Fig. 2

Three-dimensional floating culture C-MSCs can be directed towards adipogenesis/chondrogenesis but not osteogenesis due to reduced YAP/TAZ activity. ad C-MSCs were cultured in a OIM, b AIM, c CIM, or d dual medium for 5 days. Differentiation marker gene expression levels were analyzed by real-time PCR. Data were normalized to the values of C-MSCs maintained in GM. eg C-MSCs transfected with a constitutively active TAZ mutant (TAZS89A) or control plasmid were maintained in e OIM, f AIM, or g CIM for 5 days. Differentiation marker gene expression levels were analyzed by real-time PCR. Data were normalized to the values of C-MSCs transfected with control vector (cont). Values represent means ± S.D. of three cultures (**p < 0.01). All graphs are representative of three independent experiments

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