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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Type I collagen deposition via osteoinduction ameliorates YAP/TAZ activity in 3D floating culture clumps of mesenchymal stem cell/extracellular matrix complexes

Fig. 4

The effects of OIM components on COL1 production, F-actin integrity, and YAP/TAZ activity in C-MSCs. ac C-MSCs were cultured with GM (none) in the presence of β-glycerophosphate (Gly), ascorbic acid (AA), and/or dexamethasone (Dex) (Gly+AA, Gly+Dex, AA+Dex, and Gly+AA+Dex (equivalent to OIM)) for 5 days. a Confocal immunofluorescence images of COL1 (green) and nuclei (blue) in C-MSCs. Bar = 100 μm. b Confocal immunofluorescence images of YAP/TAZ (green), F-actin (red), and nuclei (blue) in C-MSCs. Bar = 20 μm. c The graph summarizes the distribution of YAP/TAZ patterns. The patterns were classified as mainly nuclear (N > C), diffuse (N = C), and mainly cytoplasmic or undetectable (N < C or undetectable)

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