Fig. 4

The effects of OIM components on COL1 production, F-actin integrity, and YAP/TAZ activity in C-MSCs. a–c C-MSCs were cultured with GM (none) in the presence of β-glycerophosphate (Gly), ascorbic acid (AA), and/or dexamethasone (Dex) (Gly+AA, Gly+Dex, AA+Dex, and Gly+AA+Dex (equivalent to OIM)) for 5 days. a Confocal immunofluorescence images of COL1 (green) and nuclei (blue) in C-MSCs. Bar = 100 μm. b Confocal immunofluorescence images of YAP/TAZ (green), F-actin (red), and nuclei (blue) in C-MSCs. Bar = 20 μm. c The graph summarizes the distribution of YAP/TAZ patterns. The patterns were classified as mainly nuclear (N > C), diffuse (N = C), and mainly cytoplasmic or undetectable (N < C or undetectable)