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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Clonal derivation of white and brown adipocyte progenitor cell lines from human pluripotent stem cells

Fig. 1

af Screening diverse cell types for BAT gene expression markers. a FABP4, b UCP1, c ADIPOQ, d LIPASIN, e CITED1, f MYH3, hES cell-derived clonal embryonic progenitor cell lines were analyzed by Illumina bead array-based gene expression for select adipocyte and BAT gene expression markers in the undifferentiated state and for 5 days in quiescence inducing conditions (Ctrl) or following adipogenic differentiation for 14 days in HyStem-C with BMP4, rosiglitazone, T3, and CL316243 (BMP4, Rosi, T3, CL) (differentiated). (C) Controls include adult BAT and SAT-derived preadipocytes and the clonal progenitor cell line E85 lacking the ability to differentiate into definitive adipocytes. Differentiated cells are identified as having definitive adipocyte markers. Values are shown as relative fluorescence units (RFUs) and represent mean values of two or more biological replicates. (RFU values < 130 considered background signal). (Error bars represent standard deviation)

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