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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Clonal derivation of white and brown adipocyte progenitor cell lines from human pluripotent stem cells

Fig. 3

Comparative staining of cell types. a Top row shows phase contrast images of BAT- and WAT-derived preadipocyte cultures and the hEP cell lines E85, C4ELS5.1, C4ELSR2, E3, NP88, and NP110 in an undifferentiated state in log growth conditions overlain with inset showing Oil red-O staining after 14 days of adipogenic differentiation in HyStem-C with BMP4, rosiglitazone, T3, and CL316243 (BMP4, Rosi, T3, CL). Bottom row shows immunofluorescent detection of UCP1 in the respective cells cultured in the above-described adipogenic media using primary rabbit anti-human UCP1 polyclonal antibody and secondary Alexa Fluor 568 donkey anti-rabbit IgG antibody. Cell nuclei were stained using DAPI. b ELISA data quantifying adiponectin in lysates of cell lines in two differentiation conditions (BMP4, Rosi, T3, CL) and (Rosi, T3, CL). (Scale bar, 100 μm)

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Stem Cell Research & Therapy

ISSN: 1757-6512