Skip to main content
Fig. 7 | Stem Cell Research & Therapy

Fig. 7

From: Clonal derivation of white and brown adipocyte progenitor cell lines from human pluripotent stem cells

Fig. 7

Extracellular flux of differentiated lines. NP88, fetal brown preadipocytes (fBAT), and subcutaneous preadipocytes (SAT) cells were differentiated in the presence of BMP4, rosiglitazone, and T3 for 14 days. Extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) were measured in real time followed by the injection of mitochondrial respiration inhibitors using Seahorse XF24 Extracellular Flux Analyzer (a and b). ATP-linked OCR (c) and proton leak OCR (d) were calculated and plotted following Seahorse XF user guide. Data represent mean ± SEM, n = 9~16; *P < 0.05***P < 0.001 Student’s t test

Back to article page