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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: DMPE-PEG scaffold binding with TGF-β1 receptor enhances cardiomyogenic differentiation of adipose-derived stem cells

Fig. 4

Following 1 or 2-week treatments with TGF-β1, ADSC cell morphology was compared in the mock, D-P, and D-P-TGF-β1 RI groups with a phase contrast microscope (A, B, a–c). ADSCs were immunostained with anti-TGF-β1 RI (green),anti-α-SMA (red), and cTnT (red). Nuclei were counterstained with DAPI (blue) (A, B, d–i). C, D The number of cells expressing TGF-β1 RI /α-SMA and TGF-β1 RI /cTnT were compared. E Western blot was performed to exam the protein expression of TGF-β1 RI, α-SMA, cTnT, p-SMad2/3, and SMad 2/3 in the mock, D-P, and D-P-TGF-β1 RI groups. G, F Comparison of relative mRNA and protein expression levels of TGF-β1 R1, α-SMA, and cTnT in the mock, D-P, and D-P-TGF-β1 RI groups (scale bar, 100 μm; values are expressed as mean ± S.D; *p < 0.05, **p < 0.01, ***p < 0.001)

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