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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: The combination of bFGF and CHIR99021 maintains stable self-renewal of mouse adult retinal progenitor cells

Fig. 2

bFGF promoted cell cycle of mNRPCs by stimulating G1/S and G2/M phase transitions. a CCK8 assay of the cells with BM, B, C, and BC medium after 1, 3, 5, and 7 days. Data are presented as mean ± SD (n = 3). b Colony formation assay of the cells with BM, B, C, and BC medium after 7 days. Data are presented as mean ± SD (n = 3). *P < 0.05; **P < 0.01 (one-way ANOVA and Sidak’s multiple comparisons test) (GraphPad Prism6). c PCA analysis of the mRNA transcriptome in mNRPCs with different treatments at various time. d, e GO analysis reveals the biological processes altered in mNRPCs on the 7th day. f, g Heatmap of RNA-seq results showed the expression of genes involved in the biological processes of cell cycle (f) and positive regulation of cell proliferation (g). h Western blot results showed that the MAPK/ERK pathway was activated in all groups and the MAPK/P38 pathway was activated in the BM, B, and C groups but not in BC. The results are representative of at least three independent experiments. Abbreviations: BM, basic medium; B, BM with bFGF; C, BM with CHIR99021; BC, BM with bFGF and CHIR99021; CFM, colony formation

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