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Fig. 8 | Stem Cell Research & Therapy

Fig. 8

From: Induced pluripotent stem cell-derived endothelial progenitor cells attenuate ischemic acute kidney injury and cardiac dysfunction

Fig. 8

Indoxyl sulfate (IS) and interleukin (IL)-1β induced cardiomyocyte apoptosis after treatment with IS or IL-1β. a Representative immunofluorescence images presenting TUNEL+ (green) HL-1 cardiomyocytes cultured in medium with or without IS or IL-1β. Con indicates control. Scale bar = 50 μm. b Bar chart depicting quantitative analyses of TUNEL+ HL-1 cardiomyocytes per high-power field (× 400 magnification). c, d Representative images of Western blot analyses and the statistical analyses for Bax, Bcl-2, and GAPDH in HL-1 cardiomyocytes cultured in medium with or without IS or IL1-β. The protein levels of Bax and Bcl-2 were normalized using GAPDH in bar charts. N = 3 per group. Data are presented as mean ± SEM. *P < 0.05 IS vs. Con, #P < 0.05 IL-1β + ΙS vs. IS, §P < 0.05 IL-1β + ΙS vs. IL-1β

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