Fig. 2

bFGF inhibits in vitro mineralization and alters osteogenic/odontogenic gene expression in SHEDs. a Expression of bFGF is reduced 50% (p < 0.05) in SHED cells during osteogenic induction over 14 days. Inclusion of 10 ng/mL bFGF in osteogenic induction medium significantly attenuates mineral deposition at 14 days (b, c) and reduces ALP enzymatic activity at 7 days (d). e–j Addition of bFGF reduces mRNA expression of RUNX2 (day 1), COL1A1 (days 1, 3, and 7), and BGLAP (day 3) (p < 0.05 for all), but does not significantly affect expression of OSX, SPP1, or DMP1. The Mann-Whitney U test was employed for comparisons of two groups. The Kruskal-Wallis test followed by pairwise comparison was used for comparison of three groups. Bars indicated the statistically significant difference (*p < 0.05, **p < 0.01). GM growth medium, OM osteogenic induction medium