Fig. 5
PPi regulates mineralization and gene expression in SHEDs. a, b Addition of 10 μM PPi to osteogenic induction medium of SHED cells nearly completely inhibits mineral deposition by 14 days. c–l At 7 days, inclusion of PPi increases COL1A1 (p < 0.05) and ANKH (p < 0.05), but does not significantly affect other genes associated with mineralization and Pi/PPi regulation, compared to untreated controls. The Mann-Whitney U test was employed for comparisons of two groups, and bars indicated the statistically significant difference (*p < 0.05). OM osteogenic induction medium