Fig. 7
Regulation of mineralization in SHEDs by continuous and short-term Pi and PPi treatment. a Cells were treated with 5 mM Pi or 10 μM PPi in osteogenic medium, with no Pi or PPi serving as negative control, and continuous administration of Pi or PPi serving as the positive control, whereas conditions A, B, and C incorporated shorter-term Pi or PPi treatments of 1, 3, or 7 days, respectively, followed by completion of the experiment in osteogenic medium. b Continuous treatment of SHEDs with Pi significantly increases (p < 0.05) mineral deposition while or continuous PPi significantly decreases (p < 0.05) mineral deposition. c Cells under condition A show no significant differences in mineralization from negative controls. d Cells under condition B exhibit PPi-mediated inhibition of mineralization by 3 days (p < 0.05). e Cells under condition C show increased mineralization from Pi treatment (p < 0.05) and decreased mineralization from PPi treatment (p < 0.05). The Mann-Whitney U test was employed for comparisons of three or more groups, and bars indicated the statistically significant difference (*p < 0.05). OM osteogenic induction medium