Skip to main content

Table 2 The differentiation capacity of CFU-F clones from human bone marrow

From: Does osteogenic potential of clonal human bone marrow mesenchymal stem/stromal cells correlate with their vascular supportive ability?

(A)
Lineage potential % of total clones (Number of clones) % of upper quartile, Tubule Length (Number) % of lower quartile, Tubule Length (Number) % of clones within group that fall within Tubule Length Upper quartile % of clones within group that fall within Tubule Length Lower quartile
AOC 64% (85) 73% (24) 50% (17) 29% 20%
OC 24% (32) 24% (8) 29% (10) 25% 31%
AO 5% (6) 0% 3% (1) 0% 17%
AC 2% (3) 0% 3% (1) 0% 33%
O 2% (3) 3% (1) 6% (2) 33% 67%
C 2% (3) 0% 6% (2) 0% 67%
Null 1% (1) 0% 3% (1) 0% 100%
(B)
Lineage potential % of total clones (Number of clones) % of upper quartile, Osteogenesis Alizarin Red (Number) % of lower quartile, Osteogenesis Alizarin Red (Number) % of clones within group that fall within Osteogenesis Alizarin Red Upper quartile % of clones within group that fall within Osteogenesis Alizarin Red Lower quartile
AOC 64% (85) 70% (23) 29% (10) 27% 11%
OC 24% (32) 21% (7) 38% (13) 22% 41%
AO 5% (6) 9% (3) 6% (2) 50% 33%
AC 2% (3) 0% 9% (3) 0% 100%
O 2% (3) 0% 6% (2) 0% 67%
C 2% (3) 0% 9% (3) 0% 100%
Null 1% (1) 0% 3% (1) 0% 100%
(C)
Lineage potential % of total clones (Number of clones) % of upper quartile, Adipogenesis Oil Red O (Number) % of lower quartile, Adipogenesis Oil Red O (Number) % of clones within group that fall within Adipogenesis Oil Red O Upper quartile % of clones within group that fall within Adipogenesis Oil Red O Lower quartile
AOC 64% (85) 91% (30) 26% (9) 35% 11%
OC 24% (32) 3% (1) 62% (21) 3% 66%
AO 5% (6) 3% (1) 3% (1) 17% 17%
AC 2% (3) 3% (1) 0% 33% 0%
O 2% (3) 0% 3% (1) 0% 33%
C 2% (3) 0% 3% (1) 0% 33%
Null 1% (1) 0% 3% (1) 0% 100%
  1. The numbers of tripotent (AOC with adipogenic, osteogenic and chondrogenic potential), bipotent (OC with osteogenic and chondrogenic potential; AO with adipogenic and osteogenic potential; or AC with adipogenic and chondrogenic potential) or unipotent (O with osteogenic potential; or C with chondrogenic potential) clones analysed are shown, with their distribution in the upper and lower quartiles of:
  2. (A) HUVEC total tubule length (TLL) following co-culture
  3. (B) Osteogenic potential as measured by Alizarin Red staining assay,
  4. (C) Adipogenic potential as measured by Oil Red O staining