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Table 2 The differentiation capacity of CFU-F clones from human bone marrow

From: Does osteogenic potential of clonal human bone marrow mesenchymal stem/stromal cells correlate with their vascular supportive ability?

(A)

Lineage potential

% of total clones (Number of clones)

% of upper quartile, Tubule Length (Number)

% of lower quartile, Tubule Length (Number)

% of clones within group that fall within Tubule Length Upper quartile

% of clones within group that fall within Tubule Length Lower quartile

AOC

64% (85)

73% (24)

50% (17)

29%

20%

OC

24% (32)

24% (8)

29% (10)

25%

31%

AO

5% (6)

0%

3% (1)

0%

17%

AC

2% (3)

0%

3% (1)

0%

33%

O

2% (3)

3% (1)

6% (2)

33%

67%

C

2% (3)

0%

6% (2)

0%

67%

Null

1% (1)

0%

3% (1)

0%

100%

(B)

Lineage potential

% of total clones (Number of clones)

% of upper quartile, Osteogenesis Alizarin Red (Number)

% of lower quartile, Osteogenesis Alizarin Red (Number)

% of clones within group that fall within Osteogenesis Alizarin Red Upper quartile

% of clones within group that fall within Osteogenesis Alizarin Red Lower quartile

AOC

64% (85)

70% (23)

29% (10)

27%

11%

OC

24% (32)

21% (7)

38% (13)

22%

41%

AO

5% (6)

9% (3)

6% (2)

50%

33%

AC

2% (3)

0%

9% (3)

0%

100%

O

2% (3)

0%

6% (2)

0%

67%

C

2% (3)

0%

9% (3)

0%

100%

Null

1% (1)

0%

3% (1)

0%

100%

(C)

Lineage potential

% of total clones (Number of clones)

% of upper quartile, Adipogenesis Oil Red O (Number)

% of lower quartile, Adipogenesis Oil Red O (Number)

% of clones within group that fall within Adipogenesis Oil Red O Upper quartile

% of clones within group that fall within Adipogenesis Oil Red O Lower quartile

AOC

64% (85)

91% (30)

26% (9)

35%

11%

OC

24% (32)

3% (1)

62% (21)

3%

66%

AO

5% (6)

3% (1)

3% (1)

17%

17%

AC

2% (3)

3% (1)

0%

33%

0%

O

2% (3)

0%

3% (1)

0%

33%

C

2% (3)

0%

3% (1)

0%

33%

Null

1% (1)

0%

3% (1)

0%

100%

  1. The numbers of tripotent (AOC with adipogenic, osteogenic and chondrogenic potential), bipotent (OC with osteogenic and chondrogenic potential; AO with adipogenic and osteogenic potential; or AC with adipogenic and chondrogenic potential) or unipotent (O with osteogenic potential; or C with chondrogenic potential) clones analysed are shown, with their distribution in the upper and lower quartiles of:
  2. (A) HUVEC total tubule length (TLL) following co-culture
  3. (B) Osteogenic potential as measured by Alizarin Red staining assay,
  4. (C) Adipogenic potential as measured by Oil Red O staining