Fig. 4

The SVF-induced proliferation of L6 myoblasts is reduced by Norleual (Nor), an HGF inhibitor. a Immunofluorescence staining of the HGF receptor c-Met (green) in L6 myoblasts, counterstained with DAPI (blue). Bar = 10 μm. b The effect of different concentrations of HGF on the proliferation rate of L6 myoblasts. c 100 pM Norleual effectively inhibits the proliferation induced by 10 ng/ml HGF. d Indirect co-culture of L6 myoblasts and SVF cells for 3 days in the presence of 100 pM Norleual. Norleual was tested on cells alone and no toxicity could be observed. e The expression of p-ERK1/2 and total ERK1/2 protein in L6 myoblasts after co-culture with SVF cells. Norleual had no effect on baseline levels of p-ERK1/2. Statistical analysis was performed using one-way ANOVA with post hoc test (Bonferroni correction). Results are presented as mean ± standard deviation. n = 3. *P < 0.05, **P < 0.01, and ***P < 0.001. ns, not significant