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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: The adipose tissue stromal vascular fraction secretome enhances the proliferation but inhibits the differentiation of myoblasts

Fig. 5

Differentiation of myoblasts into myotubes is inhibited by SVF cells and HGF. a Immunofluorescence staining of fast type myosin heavy chain (green) in C2C12 mouse myoblasts after 4 days in growth medium (GM), differentiation medium (DM), indirect co-culture with SVF cells in DM (SVF), and DM supplemented with 10 ng/ml HGF (HGF). Cells were counterstained with DAPI (blue). Bar = 200 μm. The expression of myogenic regulatory factor 5 (Myf5), slow type myosin heavy chain (Myhc1), and fast type myosin heavy chain (Myhc2) mRNA in C2C12 myoblasts after b 7 days or c 14 days of differentiation in the various culture conditions. Western blot of fast type myosin heavy chain (MyHC2) protein is also shown. β-actin was used as a loading control. Statistical analysis was performed using one-way ANOVA with post hoc test (Bonferroni correction). Results are presented as mean ± standard deviation. n = 3. *P < 0.05, **P < 0.01, and ***P < 0.001. ns, not significant

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